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Antibodies Indicated Trichinella Exposure in 24% of Wild Boar Despite No Detectable Larvae

Wild boar walking along a path in Kraków, Poland

Trichinella are food-borne zoonotic roundworms whose larvae can persist in the muscles of infected mammals. Wild boar (Sus scrofa) are important wildlife hosts, and people can become infected by eating raw or insufficiently cooked meat containing viable larvae. A study of 100 wild boar from Türkiye’s Central Black Sea Region found antibody evidence of exposure to Trichinella spp. in 24% of the animals, even though no larvae or parasite DNA were detected. The animals had been hunted in Samsun, Amasya and Çorum provinces.

Researchers used three complementary approaches. They dissolved muscle tissue so any Trichinella larvae could be concentrated and searched for under a microscope, tested meat juice for antibodies against the parasite, and used PCR to look for parasite DNA in tissue from antibody-positive animals. No larvae or parasite DNA were detected. Antibodies were found in 24% of the animals; this is the apparent seroprevalence, meaning the share that tested antibody-positive, not the share with a directly confirmed active infection.

The authors stress that the serological result indicates exposure rather than confirmed active infection. Cross-reactions with other parasitic worms can make the antibody test overestimate how many animals have been exposed. Antibody-positive animals without detectable larvae may also reflect earlier exposure, very low parasite burdens or infections that were not detectable by the direct tests at the time of sampling.

The researchers argue that combining serological screening with confirmatory methods can reveal parasite exposure that conventional larval detection alone may miss.

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